Academy Exchange

 View Only
  • 1.  HIV-1 DNA Not Detected

    Posted 08-06-2023 13:20
    Greetings-

    I have a patient who has been living with HIV since 2/2017. Treatment was started day of diagnosis. He had a change of ARV regimen due to adverse effects in 4/2021. He had an interruption in therapy due to insurance changes. Prior to restarting ARVs, HIV RNA was still not detected. This made me curious about his initial HIV test. I recently obtained HIV-1 DNA, Qual, PCR and the result was Not Detected. Is he not living with HIV? Was the initial HIV test result a false positive? Thanks in advance.hb

    --
    Hermeyone Bell, APRN, BC, AAHIVS
    Nurse Practitioner
    Bell Primary Care, LLC
    P:404-337-7486
    F: 352-329-4338


  • 2.  RE: HIV-1 DNA Not Detected

    Posted 08-06-2023 17:40
    Dear Hermoyone,
    If your patient's VL is and has been undetectable, the amount of HIV DNA (latent reservoir) is ~ 1 CD4+ cell/ million cells.
    So, detection of integrated HIV DNA often requires at least ~100+ mls of whole blood.
    David
    --
    W. David Hardy, MD
    Scientific and Medical Consultant
    Attending Physician, LA County-USC Rand Schrader (HIV) Clinic
    Adjunct Clinical Professor
    Keck School of Medicine of USC

    wdavidhardymd@gmail.com
    310-709-3505 (Mobile)





  • 3.  RE: HIV-1 DNA Not Detected

    Posted 08-07-2023 15:20

    Hermeyone,

    Yeah.  I agree.  A + HIV DNA test would help you.   But if it is negative, that really doesn't help, because the latent viral population may be  small.

    Could you find the 2017 records to confirm that that the HIV antibody test and confirmatory test, as well as the HIV RNA were positive?  Also, could you recheck a fourth gen HIV test and confirmatory antibodies?   If +, these would help you establish the presence of infection.   If negative, though, the possibility of a prior false + test, or consideration of possible seroreversion would have to be made. Not easy sometime!



    ------------------------------
    Adam Zweig
    AIDS Healthcare Foundation
    San Diego CA
    ------------------------------



  • 4.  RE: HIV-1 DNA Not Detected

    Posted 08-07-2023 15:55
    Thank you so much Drs. Hardy and Zweig. I have repeated the antibody test. His initial test was not a fourth generation and initial HIV RNA was 4400 copies. I am going to monitor him closely. Thanks again.hb

    Sent from Hermeyone's iPhone





  • 5.  RE: HIV-1 DNA Not Detected

    Posted 08-07-2023 16:49

    Good to know.   Then this may represent a case of what is called a post treatment controller.

    In any case, I would personally restart a suppressive ARV regimen.  We wouldn't want him to have viral rebound and not know it, and then put his sexual partner or partners at risk for transmission.  



    ------------------------------
    Adam Zweig
    AIDS Healthcare Foundation
    San Diego CA
    ------------------------------



  • 6.  RE: HIV-1 DNA Not Detected

    Posted 08-08-2023 15:12
    I have had success sending the HIV Qualitative DNA PCR to Quest (#8401) to confirm infection on a pediatric patient who was virally suppressed for years on meds that had negative Ag/Ab testing and negative HIV RNA PCR with scant records.

    Jennie Dougherty, MSN, RN, CPNP-AC

    Nurse Practitioner, Pediatric Infectious Diseases

    Duke Children's Hospital & Health Center


    office: 919-684-6335   /   fax: 919-668-4859  /  pager: 919-970-6525


    The information in this electronic mail is sensitive, protected information intended only for the addressee(s). Any other person, including anyone who believes he/she might have received it due to an addressing error, is requested to notify the sender immediately by return electronic mail, and to delete it without further reading or retention. The information is not to be forwarded to or shared unless in compliance with Duke Health policies on confidentiality and/or with the approval of the sender.







  • 7.  RE: HIV-1 DNA Not Detected

    Posted 08-09-2023 09:26
    Thank you so much for your input Jennie. I'm still waiting for the 4th gen result, pt. scheduled for testing tomorrow.hb

    --
    Hermeyone Bell, APRN, BC, AAHIVS
    Nurse Practitioner
    Bell Primary Care, LLC
    P:404-337-7486
    F: 352-329-4338